Fig. 4: Regulation of TM/glaucoma-related gene expression by GLIS1 in TM cells.

a Volcano plots of genes downregulated (blue) and upregulated (red) in HTM(shGLIS1) and Dox-treated HTM(pIND-GLIS1) and TM5(pIND-GLIS1) cells (as determined by DESeq2 at FDR 0.01). All other genes are in gray. Several genes associated with IOP, glaucoma, or ECM are indicated (yellow diamonds). The x-axis represents gene expression log2-fold change (FC) and the y-axis represents −log10 (p-value). b Heatmap of the differential expression of several TM-, glaucoma-, ECM-related mRNAs in HTM(shGLIS1), and HTM(Scr) (Control) cells; underlying data are rlog-transformed quantification scores as reported by DESeq2 followed by row-scaling at FDR 0.01. Data shown are for HTM (Scr), shGLIS1#1, and shGLIS1#5 replicates 1 and 2. c, d Heatmap of the differential expression of several TM-, glaucoma-, ECM-related mRNAs in HTM(pIND-GLIS1) cells (c) expressing Dox-inducible Flag‐GLIS1‐HA treated for 18 h with or without Dox (Control) (n = 2) and TM5(pIND-GLIS1) cells (n = 3) (d). e qPCR analysis of several genes downregulated in HTM(shGLIS1) compared to HTM(Scr) cells (n = 3, independent replicates). f qPCR analysis (n = 3, independent replicates) of several genes induced by murine GLIS1 in TM5 cells expressing Dox-inducible Flag‐GLIS1‐HA. Cells were treated for 18 h with or without Dox (±Dox). Data in e and f are represented as means ± SD. Statistical analyses were performed with a two-tailed Student’s t test. p Values are shown above the bars.