Fig. 2: Early PI3K activity leads to amplification of PIP₃/PIP₂ in developing ruffles, macropinosome formation, and post closure recruitment.

a Top view of an Mem-mNG isosurface rendering provides depth for 3D visualization of ruffle extension. Dual-color volumetric intensity display comparing the recruitment of AktPH-mSc to early and expanding ruffles as well as sealed macropinosomes (region 21 × 19 µm x, y). b Intensity line scan of the volumetric Mem-mNG and AktPH-mSc shows their co-scaled relative intensities for extending membrane ruffles, as well as recruitment around a sealed macropinosome. c Side view of the isosurface mesh plasma membrane and volumetric AktPH-mSc (magenta hot color scale) from a shows that the early stages of ruffle development is filled with AktPH-mSc and the resulting macropinosome (white arrow) receives a final intense AktPH-mSc recruitment around the formed macropinosome at the bottom of the ruffle (region 21 × 19 × 15 µm).