Fig. 5: The miR-106b~25 cluster suppresses cell cycle inhibitors.

a Workflow for the experiment and miRNA target validation. b Venn diagram showing the relationship between the sets of downregulated genes with miR-106b, miR-93, or miR-25 seed regions and a 1.3-fold change cut-off with respect to cel-miR-67. c A compartmentalized network of differentially expressed genes involved in cell cycle regulation. Each connection is color-coded according to the miRNA that regulates the differentially expressed gene (magenta for miR-106b, cyan for miR-93 and dark yellow for miR-25). Genes indicated by blue circles are downregulated by at least one miRNA while downregulated genes shown in blue squares are found to be bioinformatic targets by at least 1 miRNA. Genes represented by yellow circles are upregulated genes by all three miRNAs. d Heatmap representation of cell cycle regulators differentially expressed by miR-106b~25 relative to cel-miR-67. e EdU staining of rat CMs treated with selected siRNAs. f Percentage of proliferating rat CMs after siRNA treatment, mean ± s.e.m. represents n = 6 biologically independent wells examined over one independent experiment. *P < 0.05 vs corresponding control group (error bars are s.e.m.). Statistical analysis consisted of a two-tailed Student’s t-test (f). Source data are provided as a Source data file.