Fig. 6: The miR-106b~25 cluster evokes myocardial regeneration.
a Design of the study. b Real-time PCR analysis of miR-106b, miR-93 and miR-25 abundance in infarcted hearts receiving AAV9-MCS or AAV9-miR-106b~25. c Representative images of Sirius Red-stained ventricular cross-sections from the point of ligation toward the apex of hearts post-MI, treated with AAV9-MCS or AAV9-miR-106b~25. d Quantification of the infarct area of the post-infarcted left ventricles from mice receiving AAV9-MCS or AAV9-miR-106b~25. Quantification of e LVPWd, f LVIDs, and g EF of mice that received AAV9-MCS or AAV9-miR-106b~25 after sham or MI surgery. i Representative images of the infarct border zone of mice treated with AAV9-MCS or AAV9-miR-106b~25 after MI surgery, and stained for α-actinin and EdU; n refers to the number of animals. i Design of the lineage tracing study. j TdTomato expression overlaps with 5-ethynyl-2’-deoxyuridine (EdU). k Quantification of TdTomato+, EdU+ CMs. l Schematic representation of the model. *P < 0.05 vs corresponding control group; #P < 0.05 vs corresponding treatment group (error bars are s.e.m.). Statistical analysis consisted of a two-tailed Student’s t-test (b, d) or a One-way ANOVA followed by Dunnett multiple comparison test (e–g, k). Source data are provided as a Source data file.
