Fig. 2: Downregulation of GLN2 enhances ammonium tolerance. | Nature Communications

Fig. 2: Downregulation of GLN2 enhances ammonium tolerance.

From: Excessive ammonium assimilation by plastidic glutamine synthetase causes ammonium toxicity in Arabidopsis thaliana

Fig. 2

a Genomic PCR using GLN2-specific primers. g and c denote the PCR fragments derived from genomic DNA and cDNA sequences corresponding to GLN2, respectively. b Relative transcript levels of GLN2 in the shoots of 10-d-old Col, ami2, and gln2 3 d after transfer to media containing 10 mM ammonium or 10 mM nitrate (mean ± SD; n = 3). Six shoots from two plates constituted a single biological replicate. c Immunodetection of GLN1s and GLN2 isoproteins using specific antisera following SDS-PAGE and immunoblotting of total proteins from the shoots of 12-d-old Col, ami2, and gln2 5 d after transfer to media containing 10 mM ammonium or 10 mM nitrate. LSU denotes large subunits of RuBisCO. d A representative photograph of shoots from 14-d-old Col, ami2, and gln2 7 d after transfer to media containing 10 mM ammonium or 10 mM nitrate. The scale bar represents 10 mm. e FW of shoots from 14-d-old Col, ami2, and gln2 7 d after transfer to media containing 10 mM ammonium (mean ± SD; n = 8) or 10 mM nitrate (mean ± SD; n = 5). Mean values of three shoots from one plate constituted a single biological replicate. b, e Different letters denote significant differences at P < 0.05 (Tukey–Kramer’s multiple comparison test).

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