Fig. 4: Lowered activity of GLN2 reduces the conversion of ammonium to amino acids. | Nature Communications

Fig. 4: Lowered activity of GLN2 reduces the conversion of ammonium to amino acids.

From: Excessive ammonium assimilation by plastidic glutamine synthetase causes ammonium toxicity in Arabidopsis thaliana

Fig. 4

a The shoot ammonium content of 12-d-old Col, ami2, and gln2 5 d after transfer to media containing 10 mM ammonium or 10 mM nitrate (mean ± SD; n = 3). Three shoots from one plate constituted a single biological replicate. b Hierarchical clustering of the shoot amino acid content of 12-d-old Col (C), ami2 (a), and gln2 (g) 5 d after transfer to media containing 10 mM ammonium or 10 mM nitrate. The color spectrum from yellow to blue corresponds to the relative content of each amino acid. The fold-change (FC) value on each amino acid was calculated by dividing the amino acid content of a sample by the mean content of all 18 samples. Log2FC denotes the base-2 logarithm of FC. c The molar ratio of Gln to Glu in the shoots of 12-d-old Col, ami2, and gln2 5 d after transfer to media containing 10 mM ammonium or 10 mM nitrate (mean ± SD; n = 3). a, c Different letters denote significant differences at P < 0.05 (Tukey–Kramer’s multiple comparison test). b, c Six shoots from two plates constituted a single biological replicate. Three biological replicates were sampled separately three times.

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