Fig. 7: Inhibition of IL-17 and granulocyte recruitment reduces aggressive niche formation and delays tumor progression. | Nature Communications

Fig. 7: Inhibition of IL-17 and granulocyte recruitment reduces aggressive niche formation and delays tumor progression.

From: Single-cell evaluation reveals shifts in the tumor-immune niches that shape and maintain aggressive lesions in the breast

Fig. 7: Inhibition of IL-17 and granulocyte recruitment reduces aggressive niche formation and delays tumor progression.The alt text for this image may have been generated using AI.

Animals were treated with anti-IL-17/17RA or isotype control (a–f), and SX-682 diet or standard chow (g–l). a, g Schema showing experimental timeline: animals were allowed to form lesions for 1 week then treated until endpoint. All animals were collected when any one animal reached maximum tumor burden. Quantification of lesion area from animals treated with anti-IL-17/17RA or isotype control (b–d), treated in the same cohort, and SX-682 diet or standard chow (h–j), treated in the same cohort. Only mammary glands bearing lesions could be quantified. Anti-IL-17 study, n = 5 per group; SX-682 study, n = 8 per group. Pie chart showing proportion of lesions by size for Anti-IL-17 study (b) and SX-682 study (h). c, i Quantification of lesion area and number binned by size, with p value calculated by Pearson’s Chi-squared test, for Anti-IL-17 study (Chi-squared (χ2) = 10.014, df = 2) (c) and SX-682 study (Chi-squared = 29.462, df = 2) (i). d, j Stacked column graph of 20 largest lesions per group binned by size for anti-IL-17 study (d) and SX-682 study (i). P value calculated by two-sided Fisher’s exact test. e, k Percent segmented cells measured by complete blood count from peripheral blood collected at time of euthanasia and mammary gland collection. P value was calculated using two-sided Wilcoxon rank sum test; error bars indicate standard error of the mean (SEM). Each dot represents one animal. e Anti-IL-17 group, n = 7; control group, n = 32 pooled from multiple identically treated control cohorts; U = 43. k SX-682 group, n = 20; control group, n = 29 pooled from multiple identically treated cohorts, U = 169.5. f, l Kaplan–Meier plot showing tumor-free survival curves of treated animals. Two-sided p value calculated using log-rank (Mantel–Cox) test. f Anti-IL-17 group, n = 7; control group, n = 32 pooled from multiple identically treated cohorts. l SX-682 group, n = 20; control group, n = 29 pooled from multiple identically treated cohorts. Source data are provided as a Source Data file.

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