Fig. 2: Scalability and comparison of DL4-μbeads to plate-bound DL4-Fc cultures.

a 1 × 10³ CB-CD34⁺ cells were cultured with DL4-μbeads or on plate-bound DL4-Fc, as indicated, for 14 days and analyzed by flow cytometry for the expression of CD5 and CD7. Cell counts were normalized to input day 0 CB-derived CD34⁺ cells (n = 3). b CB-CD34⁺ cells were placed in a limiting dilution assay for 14 days before scoring for the presence of CD45⁺ CD7⁺ CD56⁻ cells. Statistical analysis was performed via the method of maximum likelihood applied to the Poisson model. c DL4-μbeads were assessed for adaptability to G-Rex system. 2.5 × 10⁴ CB-CD34⁺ cells were cultured with DL4-μbeads in a G-Rex 24-well plate format for 7 days, followed by a full-media change and re-addition of fresh DL4-μbeads for another 7 days. D14 cultures were harvested, counted and analyzed for the expression of CD34, CD7 and CD5, as indicated, from CD45⁺ -gated cells. Cell counts were normalized to input day 0 CB-derived CD34⁺ cells (n = 3). PB = plate-bound. Data represent means ± s.d. of n independent experiments. (P > 0.05); *P < 0.05 (one-way ANOVA followed by Fisher’s Least Significant Difference). G-Rex image was created with BioRender.com (Toronto, ON).