Fig. 7: Cholinergic enhancement of CA1 responses to temporoammonic inputs is mediated by inhibition. | Nature Communications

Fig. 7: Cholinergic enhancement of CA1 responses to temporoammonic inputs is mediated by inhibition.

From: Acetylcholine prioritises direct synaptic inputs from entorhinal cortex to CA1 by differential modulation of feedforward inhibitory circuits

Fig. 7

A, B Inhibition of GABAA receptors with picrotoxin (50 µM) reduced the underlying hyperpolarising envelope (red) in response to 10 stimuli at 10 Hz for both SC (green; control, n = 15 from 5 mice; GABAA antagonist, n = 7 from 2 mice; p = 0.028, two-tailed unpaired t-test) and TA (purple; control, n = 23 from 9 mice; GABAA antagonist, n = 7 from 2 mice; p = 0.021, two-tailed unpaired t-test) input pathways to CA1 pyramidal neurons. C–E In the presence of a GABAA receptor antagonist, light stimulation of cholinergic fibres (LED) reduced spike probability for SC pathway but produced no change in the TA pathway (SC spike probability, n = 13 from 7 mice, p = 0.005; TA spike probability, n = 12 from 6 mice, p = 0.38; SC vs TA spike probability, p = 0.37; inter-group comparisons one-way ANOVA with post hoc Bonferroni correction. Within-group comparisons two-tailed paired t-test). Data are mean ± SEM; **p < 0.01, *p < 0.05. Source data are provided as a Source Data file.

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