Fig. 2: Membrane mixing upon SNARE-mediated fusion.
From: En route to dynamic life processes by SNARE-mediated fusion of polymer and hybrid membranes
a The nearly neutral zeta potential of lipid (Lip), polymer (Poly), and hybrid (Hyb) vesicles promotes membrane mixing. The surface charge, introduced by lipid dyes was neutralized with KCl. b–d Comparison of SNARE-induced and spontaneous membrane mixing in different amphiphiles. Mean values of at least two different reconstitutions with standard deviations are shown. Inset of (d): Variability of SNARE-mediated membrane mixing in polymersomes. Individual measurements, as well as their mean value with standard deviation, are shown. e–g Intensity-based size distribution with indicated peak values of different vesicles upon synaptobrevin (Syb) or ΔN complex (ΔN) reconstitution before and after fusion. Size increase corresponding to several rounds of fusion can be seen in the lowest panels.
