Fig. 4: Adjustment of vitrified ice thickness by tuning pressure and choosing EM grids with different carbon hole sizes.
From: A simple pressure-assisted method for MicroED specimen preparation
a-d Representative low magnification TEM images and typical ED patterns taken from orthorhombic lysozyme crystal specimens prepared under different pressures (17.2 and 27.7 mbar) and Quantifoil grids with different hole sizes (R 1.2/1.3 and R 3.5/1). Insets display the areas of lysozyme crystals (marked by red circles) from which the diffraction patterns were taken. This comparison shows both the pressure and hole size of the grid play important roles in ice thickness adjustment. Each specimen preparation condition was repeated three to five times and the results are similar.
