Fig. 5: Examples of specimens prepared successfully by Preassis from non-viscous to viscous buffer conditions.
From: A simple pressure-assisted method for MicroED specimen preparation
a MicroED specimens prepared from tetragonal lysozyme crystal sample under non-viscous condition (without high molecular weight polymer in the buffer) using grid R 1.2/1.3 and pressure of 37.2 mbar. b, c MicroED specimens prepared from R2lox and GTPase crystal samples under viscous condition with 44% PEG 400 and 30% PEG 4000, respectively. The pressure used for R2lox specimen is 30.7 mbar, and GTPase is 181 mbar. Grids R 3.5/1 were used for both experiments. ED patterns were taken from the crystal areas highlighted by red circles. Each specimen preparation was repeated at least three times and the results are similar.
