Fig. 6: Nuclease null Cas9 variants have similar sequence preferences in the PAM proximal 2 bp.

Using a dCas9 dataset from Horlbeck et al. 2016³⁹ and base editing datasets from Marquart et al. 2020⁴⁰, we grouped gRNA by the PAM proximal 2 bp, averaged activity and correlated the grouped averages with the datasets from Fig. 5.