Fig. 1: Genes encoding BATF3 and subunits of the IL-2R are highly acetylated at H3K27 and positively correlate. | Nature Communications

Fig. 1: Genes encoding BATF3 and subunits of the IL-2R are highly acetylated at H3K27 and positively correlate.

From: Super-enhancer-based identification of a BATF3/IL-2R−module reveals vulnerabilities in anaplastic large cell lymphoma

Fig. 1: Genes encoding BATF3 and subunits of the IL-2R are highly acetylated at H3K27 and positively correlate.

a Enhancers were ranked based on increasing H3K27ac signals. Genes within SEs in Karpas-299 (ALK+) and Mac-1 (ALK−) ALCL cell lines are marked red. b H3K27ac ChIP-seq tracks at the BATF3 locus with indicated SE regions. c Overlap of genes associated with SEs in Karpas-299 and Mac-1 cell lines. d Enhancers were ranked based on increasing H3K27ac signals. Genes within SEs in primary ALCL patient samples (#54 and #208) are marked in red. e Identification of upstream regulators among deregulated genes in CRISPR/Cas9-mediated BATF3-KO Karpas-299 cells by IPA 2020 analysis. f Analysis of BATF3-correlated genes in a previously published RNA-seq dataset of 23 ALCL patients (BioProject PRJNA255877, SRA identifier SRP044708). Pearson correlation; P value based on t-distribution. g Representative images of 63 patient samples measured of BATF3 and IL-2Rα IHC staining of ALCL, ALK+ in paired FFPE tissues sections. h IHC quantification of IL-2Rα, IL-2Rβ and IL-2Rγ expression in mature T-NHL TMAs (reactive lymph node controls, n = 11; AITL, n = 8; PTCL-NOS, n = 23; ALCL, ALK+, n = 22; ALCL, ALK−, n = 23) and pcALCL (n = 24) specimens. P values were determined by two-tailed unpaired Student’s t test. All box-whisker plots represent the median (central line), 25th–75th percentile (bounds of the box) and minimum–maximum (whiskers).

Back to article page