Fig. 6: Hepatic p53 mediates the gluconeogenic action of adrenaline and cortisol.
From: O-GlcNAcylated p53 in the liver modulates hepatic glucose production
a p53 levels in THLE-2 cells in the presence or absence of adrenaline (n = 5). b p53 levels in THLE-2 cells kept in complete medium (CM) or KHH + adrenaline in the presence or absence of OSMI-1 (n = 6). c Protein levels of p53 and PCK1 and d PCK1 activity in THLE-2 cells transfected with empty-siRNA or siRNA-p53 and then treated with KHH alone (n = 5) or KHH + adrenaline (n = 7). e PCK1 levels in Hep3B cells maintained in CM or KHH in the presence or absence of adrenaline (n = 5). f Blood glucose levels in p53 floxed mice injected with AAV8 expressing either GFP or Cre and then treated with saline or adrenaline (n = 6); * depicts differences compared to AAV8-GFP + saline, # depicts differences between AAV8-GFP and AAV8-Cre mice treated with adrenaline. g Blood glucose levels in p53 LKO mice and their control littermates treated with saline or adrenaline (n = 6). * depicts differences compared to control + saline, and # depicts differences between control and LKO mice treated with adrenaline. h p53 levels in THLE-2 cells incubated with different cortisol concentrations (n = 5). i p53 levels in THLE-2 cells kept in CM or KHH + hydrocortisone in the presence or absence of OSMI-1 (n = 3). j Protein levels (n = 6) of PCK1 and p53 and k PCK1 activity (n = 5) in THLE-2 cells transfected with empty-siRNA or siRNA-p53, and then treated with hydrocortisone. l PCK1 levels in starved Hep3B cells with different cortisol concentrations (n = 5). m Blood glucose levels in p53 floxed mice injected with AAV8 expressing either GFP or Cre and then treated with saline or hydrocortisone (n = 7 and 10). n Blood glucose levels in p53 LKO mice and their control littermates treated with saline or hydrocortisone (n = 7 and 12). Expression of GAPDH served as a loading control, and control values were normalized to 100%. Dividing lines indicate splicings within the same gel. Data were presented as mean ± standard error mean (SEM). * or # denotes P < 0.05, ** denotes P < 0.01, and *** denotes P < 0.001, determined in all cases by one-way ANOVA followed by Bonferroni post hoc testing. “n” denotes independent animals or cell culture wells. Source data are provided as a Source Data file.
