Fig. 8: Hepatic p53 affects postprandial hyperglycemia and is elevated in models of insulin resistance.

a Hepatic OGT and p53 protein levels in WT mice fed with standard diet or high-fat diet (HFD) 60% for 4 days (n = 7). Hepatic OGT protein levels in (b) control mice and p53 LKO mice fed with HFD 60% for 4 days (n = 6) and c FLOXp53 mice injected with AAV8 expressing either GFP (n = 7) or Cre (n = 5) and fed with HFD 60% for 4 days. Blood glucose levels in Alfp-Cre± mice injected with AAV-DIO expressing either GFP or p53 refed with d chow diet (n = 11 and 12) and e HFD after overnight fasting (n = 11 and 12). Blood glucose levels in FLOXp53 mice injected with AAV8 expressing either GFP or Cre refed with f chow (n = 6 and 8) diet and g HFD after overnight fasting (n = 5 and 6). Blood glucose levels in control and p53 LKO (n = 6) mice refed with h chow (n = 5 and 7) diet and i HFD (n = 7) after overnight fasting. j Hepatic p53 and PCK1 protein levels in WT (n = 4) and db/db mice (n = 9). The area under the curve (AUC) is provided. Expression of GAPDH served as a loading control and control values were normalized to 100%. Dividing lines indicate splicings within the same gel. Data were presented as mean ± standard error mean (SEM). * denotes P < 0.05, ** denotes P < 0.01, and *** denotes P < 0.001, determined by two-tailed Student’s t-test. “n” denotes independent animals. Source data are provided as a Source Data file.