Fig. 2: Cycloheximide (CHX) does not affect the translation ramp or global translation levels. | Nature Communications

Fig. 2: Cycloheximide (CHX) does not affect the translation ramp or global translation levels.

From: Humans and other commonly used model organisms are resistant to cycloheximide-mediated biases in ribosome profiling experiments

Fig. 2

a Normalized ribosomal A-site coverage observed in long footprints (29–31 nt) for the first 200 (left) and last 200 (right) codons in HEK 293T cells in highly expressed genes (>64 reads). The solid line depicts the mean and shaded areas represent 95% confidence intervals for three biological replicates (n = 3). b Differential ribosome occupancy of open reading frames (ORFs) in HEK 293T cells across inhibitor treatments were identified using DESeq2, which uses a negative binomial distribution model with fitted mean47. Uniquely mapped reads were split into short footprints (bottom left; 21–22 nt) and long footprints (top right; 28–32 nt). ORFs, after excluding the first 15 codons, were tested for differential translation (adjusted p-value ≤ 0.05) and for unaltered translation (adjusted p-value ≤ 0.05). Significantly altered ORFs are indicated in blue, unaltered transcripts in red.

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