Fig. 3: Cycloheximide (CHX) does not alter mammalian ribosome occupancy at the A-site and the P-site. | Nature Communications

Fig. 3: Cycloheximide (CHX) does not alter mammalian ribosome occupancy at the A-site and the P-site.

From: Humans and other commonly used model organisms are resistant to cycloheximide-mediated biases in ribosome profiling experiments

Fig. 3

a Correlation analysis of transcriptome-wide A-site codon occupancy in HEK 293T cells across different CHX treatments for short and long footprints. The solid line depicts the fitted line and shaded areas represent 95% confidence intervals for three biological replicates (n = 3). Each black dot represents a codon. The size of the box indicates p-values. Correlations with a p-value > 0.05 are crossed out. b Like a for P-site codon occupancy. c Like a for E-site codon occupancy. Footprints were excised between 18 and 32 nt but are represented according to size: short footprints (21–22 nt) and long footprints (29–31 nt).

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