Fig. 4: KU-60019 increases 1-bp insertions at multiple native human genomic loci, and hundreds of pathogenic alleles can be created or repaired using 1-bp insertions.
From: Small molecule inhibition of ATM kinase increases CRISPR-Cas9 1-bp insertion frequency
Fraction of SpCas9-NG 1-bp insertion outcomes among all edited reads at noted Precision50 genomic loci in U2OS (a) and HCT-116 (b), N = 2 biologically independent samples. Histogram of inDelphi-predicted fraction of major cutsite-proximal SpCas9 editing outcomes that should lead to c repair to wild-type genotype for pathogenic 1-bp deletion variants from the ClinVar and HGMD databases; d installation of pathogenic 1-bp insertion variants from the ClinVar and HGMD databases using inDelphi models trained on cell types as listed in legend.
