Fig. 2: Characterization of dynamic response events underlying single-cell responses.

a Single neuron RDI as a function of responsiveness (session-average z-score of ΔF/F activity). Each colored dot is one neuron; black dots are 10th percentile binned means; black line is a linear fit of the binned data; shaded area indicates 95th percent confidence interval of the linear fit. Neurons are z-scored using the entire recording on a given session. Data are shown for all neurons responsive to both PDG and MOV (n = 736 neurons). b Response events from one example neuron. Top: ΔF/F responses, all trials across all recording sessions. Bottom: Trial-averaged response. Shaded areas indicate identified events. Insets: z-score trajectories (smoothed using 30-point moving average) across all trials for the three events in the example neuron. c Number of response events per neuron. Left: all PDG responsive neurons are shown in gray, dual-responsive neurons shown in color. Right: all MOV responsive neurons shown in gray, dual-responsive neurons shown in color. d Proportions of event types (growing, decaying, and static) in responses to both stimuli. Event type is determined by z-scoring an event waveform’s single-trial responses and comparing the distributions of these values between the first two sessions (60 trials) and the last two sessions (60 trials; Wilcoxon rank-sum test). Gray dots are individual fields. Bar data shown are mean proportions across all imaging fields ± sem (n = 13 imaging fields; growing events t₁₂ = −2.6, p = 0.04; decaying events t₁₂ = −4.2, p = 0.001; static events t₁₂ = 4.8, p = 3.8 × 10⁻⁴; two-tailed paired-samples t-test; *p < 0.05, **p < 0.01, ***p < 0.001). e Visualization of MOV response event magnitude changes. Left: bands indicate event periods for each neuron, colored by event type. Neurons are ordered by time of maximum trial-averaged response. Right: proportions of event types for neurons’ peak responses (diagonal of the left plot). Data are shown for all dual-responsive neurons. f Event instability (normalized delta z-score) as a function of event magnitude (session-average event z-score). Each colored dot is one event; black dots are 10th percentile binned means ± 95th percent confidence interval. Box plots are the first quartile of data tested against the fourth quartile (n = 365 events per quartile for PDG, Z = 7.3; n = 441 events per quartile for MOV, Z = 3.4; ***p < 0.001, two-sided Wilcoxon ranksum test). Data are shown for all dual-responsive neurons. Boxplots are centered on the median, boxes extend to first and third quartiles, whiskers extend to 1.5 times the interquartile range or minima/maxima in the absence of outliers. g MOV events are less stable than PDG events independent of event magnitude. Binned means (10th percentiles) using data from (f) for both stimuli shown together. Data from all events across both stimuli were pooled to determine bin edges, events from each stimulus were then binned separately. Error bars represent the 95th percent confidence interval, gray lines are linear fits of the data. Significance markers indicate comparison of PDG and MOV values in each bin (**p < 0.01 for first bin, ***p < 0.001 for all other bins; two-sided Wilcoxon rank-sum test).