Fig. 6: D2-MSNs in aDLS regulate habitual actions.

a Graphical representation of sensory-specific satiety-induced devaluation procedure used to assess the goal-directedness of lever-pressing behavior. Mice were pre-fed with chow (valued condition) or pellets (devalued condition) (1), then permitted to lever-press under extinction conditions (2). b Mean (±s.e.m.) number of lever presses under extinction conditions in mice expressing hM4Di in aDLS (n = 22) trained under an RI60 schedule after vehicle or CNO injection in valued and devalued conditions; F_(1,16) = 13.84, p = 0.0019, interaction between Value and CNO in Two-way repeated measures ANOVA; **p = 0.0035, post-hoc test. c Numbers of bouts of lever presses (±s.e.m.); F_(1,16) = 17.56, p = 0.0007, interaction between Value and CNO in Two-way repeated measures ANOVA; **p = 0.0017, post-hoc test. d Mean (±s.e.m.) number of magazine entries (±s.e.m.); F_(1,16) = 1.807, p = 0.1977, interaction effect in Two-way repeated measures ANOVA. e Mean (±s.e.m.) number of lever presses under extinction conditions in D1-Cre mice expressing hM4Di in aDLS (n = 8) trained under an RI60 schedule after vehicle or CNO injection in valued and devalued conditions; F_(1,7) = 0.124, p = 0.7351; interaction effect in Two-way repeated measures ANOVA. f Mean (±s.e.m.) number of bouts of lever presses; F_(1,7) = 0.1076, p = 0.7525. g Mean (±s.e.m.) number of lever presses under extinction conditions in D2-Cre mice expressing hM4Di in aDLS (n = 8) trained under an RI60 schedule after vehicle or CNO injection in valued and devalued conditions; F_(1,7) = 0.6.359, *p = 0.0397; interaction effect in Two-way repeated measures ANOVA. h Mean (±s.e.m.) number of bouts of lever presses; F_(1,7) = 6.055, *p = 0.434; interaction effect in Two-way repeated measures ANOVA.