Fig. 6: Nkcc1 overexpression in OLs enhances long-term potentiation and hippocampal-dependent learning. | Nature Communications

Fig. 6: Nkcc1 overexpression in OLs enhances long-term potentiation and hippocampal-dependent learning.

From: Oligodendrocytic Na+-K+-Cl– co-transporter 1 activity facilitates axonal conduction and restores plasticity in the adult mouse brain

Fig. 6: Nkcc1 overexpression in OLs enhances long-term potentiation and hippocampal-dependent learning.

a Induction of OL-specific Nkcc1 overexpression. b Recording of excitatory postsynaptic currents (EPSCs) from bursting neurons in the subiculum and electrical stimulation protocol of theta burst stimulation for the induction of long-term potentiation. c Changes in EPSC amplitude induced by theta burst stimulation with different numbers of bursts in wild-type (WT) mice (10 bursts, n = 7; 15 bursts, n = 7; 20 bursts, n = 6, PND 36–43) and mice overexpressing Nkcc1 (NKCC1-OE) in OLs (10 bursts, n = 8; 15 bursts, n = 7; 20 bursts, n = 8, PND 38–58). Data are presented as mean ± SEM. d Summary histograms for the change in EPSC amplitude at 35–40 min after theta burst stimulation. Data for 5 bursts (WT, n = 5; NKCC1-OE, n = 9) are additionally shown. Two-way ANOVA, F(1,47) = 7.17, P = 0.010. Data are presented as mean ± SEM. *P < 0.05. e OL-specific Nkcc1 overexpression in a stage-specific manner. NKCC1-OE was induced by removing doxycycline (Dox)-containing chow at 3 weeks before starting the contextual fear conditioning (CFC) test. f Experimental paradigm for the CFC test. g, h Percentage of freezing time in the CFC test (control mice, n = 14; NKCC1-OE mice, n = 15) at conditioning (Day 1) (g) and at contextual test (Day 2) (h). Unpaired two-sided Student’s t test, t27 = 3.69, P = 0.0010 (left); two-way repeated-measures ANOVA, F(1, 144) = 33.5, P < 0.0001 (right). Data are presented as mean ± SEM. *P < 0.05. Source data are provided as a Source Data file.

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