Fig. 2: Correlation between intra- and inter-tumor heterogeneity of epithelial cells.
From: Dissecting esophageal squamous-cell carcinoma ecosystem by single-cell transcriptomic analysis
a tSNE plot of 44,122 epithelial cells, colored by cluster. Only tumors with at least 100 epithelial cells are shown. b Pie charts showing the proportions of cells originating from each patient detected in each cluster, colored by patient. Clusters are ordered by the proportion of the most dominant patient and are classified into Group 1 cluster or Group 2 cluster. c Stacked histograms showing proportions of epithelial cell clusters for each patient, colored by cluster as in (a). d Schematic showing the methods for quantifying the intra-tumor (solid line) and inter-tumor (dashed line) heterogeneities of epithelial cells for each patient based on the patient average (yellow star) and global average (red star). e Scatterplot showing the positive correlation between the intra- and inter-tumor heterogeneities. Two-sided Spearman correlation coefficient and P value are indicated. Shaded region indicates 95% confidence interval for the correlation. f Boxplot showing the relative inter-tumor heterogeneity levels for patients with Group 1 cluster (green) or without Group 1 cluster (yellow). Boxplots show the median (central line), the 25–75% interquartile range (IQR) (box limits), the ±1.5 times IQR (Tukey whiskers).
