Fig. 5: WT ∆133p53β interaction with p63 family members modifies cancer cell invasive capacities. | Nature Communications

Fig. 5: WT ∆133p53β interaction with p63 family members modifies cancer cell invasive capacities.

From: Δ133p53β isoform pro-invasive activity is regulated through an aggregation-dependent mechanism in cancer cells

Fig. 5: WT ∆133p53β interaction with p63 family members modifies cancer cell invasive capacities.

a RT-PCR analysis of p63 isoform expression in different cancer cell lines. n = 3. b Co-immunoprecipitation analysis of GFP- WT Δ133p53β interaction with ΔNp63α or ΔNp63γ in MCF-7 cells upon co-expression. n = 2. c Invasion of MCF-7 breast cancer cells expressing Δ133p53β, ΔNp63α or ΔNp63γ either alone or in combination as indicated. Invasion assays were performed for 48 h upon protein expression. The results are expressed as the fold change compared to control. Values are the mean of ±SEM (error bars) of five independent experiments. Statistical analysis was performed using non-parametric Mann–Whitney t test with the Prism software (GraphPad). p values are 0,0075, 0,0317 and 0,0159 respectively in the order of the appearance in the figure. d. Western blot analysis of E- and N-Cadherin in MCF-7 cells expressing GFP-tagged Δ133p53β, ΔNp63α or ΔNp63γ, either alone or in combination as indicated. n = 2. e Western blot analysis of ΔNp63 depletion upon Sh RNA application in MCF-7 cell line. Representative immunoblot of one out of 3 experiments from Fig. 5f is shown. f Invasion of MCF-7 breast cancer cells expressing with Δ133p53β in control and ΔNp63 depleted background as indicated. MCF-7 cells were assayed for 48 h and the changes in the invasion were analysed as in (c). The results are expressed as the fold change ratio compared to control. The values plotted are means ± SEM of four independent experiments. Statistical analysis was performed using non-parametric Mann–Whitney t test with the Prism software (GraphPad). p values are 0.0211 and 0.286. g Invasion of MCF-7 breast cancer cells in TAp63 or double depleted (TAp63 and Δ133p53 and TAp63 and β) background as indicated. MCF-7 cells were assayed for 48 h and the changes in invasion were analysed as in (c). The results are expressed as the fold change ratio compared to control. The values plotted are means ± SEM of four independent experiments. Statistical analysis was performed using non-parametric Mann–Whitney t test with the Prism software (GraphPad). h Invasion of MDA-MB-231 D3H2LN breast cancer cells under conditions of overexpression of TAp63α. Cells were assayed for 48 h and the changes in invasion were analysed as in (c). The results are expressed as the fold change ratio compared to control. The values plotted are means ± SEM of four independent experiments. Statistical analysis was performed using non-parametric Mann–Whitney t test with the Prism software (GraphPad). p value is 0.0211. i Invasion of MDA-MB-231 D3H2LN breast cancer cells in TAp63α and Δ133p53 depleted background as well as in double depleted background. Cells were assayed for 48 h and the changes in invasion were analysed as in (c). The results are expressed as the fold change ratio compared to control. The values plotted are means ± SEM of four independent experiments. Statistical analysis was performed using non-parametric Mann–Whitney t test with the Prism software (GraphPad). The first p value is 0,0061 while the other two are <0.0001. Molecular weight is expressed in kDa. Source data are provided as a Source Data file.

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