Fig. 4: Anti-CD4 post conditioning enriches IL-18Rα^hi endogenous CD8⁺ T cells.

a Schematic of the experiment. Injected cell number: B16-F10, 2 × 10⁵/mouse; ex vivo-primed Thy1.1⁺ Pmel-1 CD8⁺ T (ex-T) cells, 2 × 10⁶/mouse. b Differentially expressed genes in microarray analysis. Genes that were upregulated (>3-fold) in CTX^pre/CD4^post compared with that in CTX^pre-experienced endogenous CD8⁺ T (en-T) cells are indicated. En-T cells isolated as in Supplementary Fig. 3f were used. For each group, en-T cells from five mice were pooled and used for analysis. c IL-18Rα expression in en-T cells was analyzed. n = 5 mice per group. Two-tailed unpaired Student’s t-test. d–f Analysis of TCR distribution and gene expression profiles of IL-18Rα^hi en-T cells. d Schematic of analyte preparation. Injected cell number: B16-F10, 2 × 10⁵/mouse; ex-T cells, 2 × 10⁶/mouse. On day 25, IL-18Rα^hi-enriched en-T cells were isolated via magnetic cell separation. e Shannon evenness (normalized Shannon–Wiener index) of TCR α-chain (left) and β-chain (right) are shown. n = 3 mice per group. f Whole transcriptome analysis. Genes that were upregulated (>3-fold) in IL-18Rα^hi-enriched en-T cells compared with en-T cells in the flow-through are indicated. n = 4 mice per group. g Multi-color flow cytometry analysis of en-T cells. Analytes prepared as in a were stained with the indicated markers and analyzed. Dimensionality reduction (t-SNE; t-distributed stochastic neighbor embedding) was performed for visualization. c, e Each symbol indicates an individual mouse. Horizontal bars indicate means. xCD4^post, anti-CD4 post conditioning; CTX^pre, cyclophosphamide preconditioning. Source data are provided as a Source Data file.