Fig. 2: Interaction between Kapβ2 and PR poly-dipeptides. | Nature Communications

Fig. 2: Interaction between Kapβ2 and PR poly-dipeptides.

From: C9orf72-derived arginine-rich poly-dipeptides impede phase modifiers

Fig. 2: Interaction between Kapβ2 and PR poly-dipeptides.The alternative text for this image may have been generated using AI.

a Immunoprecipitation showing interaction between endogenous Kapβ2 and HA:SBP:GFP:PR20:HA expressed in HeLa cells. b Pull-down binding assay showing interaction between GST:Kapβ2 and MBP:PR18/MBP:PR8. c Dissociation constant (Kd) measured by ITC of Kapβ2 (ΔLoop) binding to MBP:PR18. d SEC-MALS of Kapβ2 in the absence and presence of MBP:PR18, showing a 1 : 1 complex formation between Kapβ2 and MBP:PR18. The experiments (a, b) were independently repeated three times or more with similar results. Source data are provided as a Source Data file.

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