Fig. 5: The model of interaction between PR poly-dipeptides and Kapβ2.
From: C9orf72-derived arginine-rich poly-dipeptides impede phase modifiers
a Electrostatic potential of Kapβ2 and MD calculated model of PR poly-dipeptide inside of Kapβ2 cavity are shown. Blue and red colors show the positively charged and negatively charged regions, respectively. PR poly-dipeptide is colored blue. b Close-up view of Kapβ2 cavity. Methyl groups located close to the Kapβ2 cavity, isoleucine, leucine, valine, and methionine, are represented as spheres colored green, blue, yellow, and orange, respectively. Amino acids within 5 Å around the PR poly-dipeptide are colored cyan. c Pull-down binding assay showing the competition of PR20 and NLS of FUS to GST:Kapβ2. The experiment was independently repeated twice with similar results. Source data are provided as a Source Data file.
