Table 1 Immunostaining conditions.
Quenching | Blocking | Primary | Secondary | |
|---|---|---|---|---|
IBA1 | 2% H2O2 in 70% methanol for 10 min | 10% FCS + 3% BSA + 0.5% Triton, in Tris-buffered saline for 1 h at room temperature | 1:800 in blocking buffer O/N at 4 °C | 1:500 anti-rabbit antibody in Tris-buffered saline with 0.5% Triton |
CSF1R | 0.3% H2O2 for 10 min | 10% NGS + 0.2% Triton X-100 in PBS at room temperature for 1 h | 1:250 in blocking buffer O/N at 4 °C | 1:500 goat anti-rabbit in PBS + 0.2% Triton X-100 for 1.5 h at room temperature |
0.1% NaBH4 for 30 min | ||||
CD13 | – | 1% BSA + 2% Triton in phosphate-buffered saline for 1 h at room temperature | 0.8 μg/mL in blocking buffer O/N at 4 °C | 1:500 anti-goat in phosphate-buffered saline |
AQP4 | – | 10% NGS + 0.1% Triton in phosphate-buffered saline for 1 h at room temperature | 1:400 in blocking buffer O/N at 4 °C | 1:500 anti-rabbit in phosphate-buffered saline |
CD206 | 2% H2O2 in 70% methanol for 10 min | 10% FCS + 3% BSA + 0.05% Triton, in Tris-buffered saline for 1 h at room temperature | 1:300 in blocking buffer O/N at 4 °C | 1:500 anti-rat antibody in Tris-buffered saline with 0.05% Triton |
P2RY12 | – | 10% NGS + 0.4% Triton, in Tris-buffered saline for 1 h at room temperature | 1:400 in blocking buffer O/N at 4 °C | 1:500 anti-rabbit antibody in Tris-buffered saline |
CD31 | – | 5% BSA + 0.3% Triton in phosphate-buffered saline for 1 h at room temperature | 1:150 in blocking buffer O/N at 4 °C | 1:500 anti-Armenian hamster in phosphate-buffered saline with 0.3% Triton |
