Fig. 5: Isobutene production by TaFdc and AnFdc variants.
From: Directed evolution of prenylated FMN-dependent Fdc supports efficient in vivo isobutene production
3-methylcrotonate decarboxylation assay with purified enzyme comparing isobutene production as detected by GC by TaFdc and AnFdc variants (Table 1), both with N-terminal His-tags, over 2 and 4 h with 10 mM 3-methylcrotonate and 0.3 mg/mL enzyme. Fold increase comparison is shown in Supplementary Fig. 7. Source data are provided as a Source Data file.
