Fig. 2: EPR characterizes binding of the metal ion co-factor and ADP:AlF4− to DnaB. | Nature Communications

Fig. 2: EPR characterizes binding of the metal ion co-factor and ADP:AlF4− to DnaB.

From: Spectroscopic glimpses of the transition state of ATP hydrolysis trapped in a bacterial DnaB helicase

Fig. 2

a EDNMR highlighting hyperfine couplings and thus proximities between the Mn2+ metal center and surrounding nuclei measured for DnaB:ADP:AlF4− (red) and DnaB:ADP (cyan), as well as for a control solution containing only Mn2+:ADP:AlF4− in the same buffer used for the protein sample (purple). The assignments of the peaks to the nuclear resonance frequencies are shown. The cyan spectrum is reproduced from ref. 64. Asterisks mark a broad, currently unassigned feature that is possibly due to Mn double quantum or combination lines23. 31P Davies ENDOR (b) and Mims ENDOR (c) recorded on DnaB:ADP:AlF4−. The red lines represent line shape simulations using EasySpin106 based on Aiso-values of 0.3 and 4.7 MHz (for all parameters see Supplementary Table 1). The broad background peak in (b) is most likely a third harmonic of one of the Mn2+ hyperfine lines and was removed for fitting.

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