Fig. 3: Allele-specific expression is established during differentiation and then maintained.
From: Locus specific epigenetic modalities of random allelic expression imbalance
a Analysis by RT-PCR followed by pyrosequencing of Acyp2 and Bag3 allelic expression ratio in 18 clones generated from the F1-21.6 ESC line (left panels). Comparison of the cumulative distributions of allelic expression ratios in ESCs and NPCs (Kolmogorov–Smirnov tests, right panels). n is the number of clones analyzed. b Comparison of the allelic expression ratios of the genes previously categorised as RME in NPCs11 and after differentiation into astrocytes, for five NPC clones, measured by allele-specific RNA-seq. The number of assessable genes per clone (values with less than 20 reads were excluded) is nXX1 = 75, nXX2 = 146, nXX3 = 121, nXY1 = 150, nXY2 = 137. Pearson correlation coefficients were calculated for each NPC clone. c Comparison of the allelic expression ratio for the nine genes, which showed several modalities of expression, in five NPC clones and after differentiation into astrocytes.
