Fig. 6: OPTN deficiency impairs recruitment of CD4 and CD8 lymphocytes to the CNS.
From: OPTN is a host intrinsic restriction factor against neuroinvasive HSV-1 infection
Principal component analysis of cytokine protein expression profiles from 8dpi (n = 5 mice per group) or mock infected (n = 3 mice per group) Optn+/+ or −/− mice to compare (top) PC1 vs PC2 or (bottom) PC1 vs PC3 are shown for a draining lymph nodes and b brainstems. Brainstem cell suspensions from mock infected Optn+/+ (n = 4 mice), mock infected Optn−/− (n = 5 mice), 8dpi Optn+/+ (n = 10 mice), and 8 dpi Optn −/− (n = 8 mice) were analyzed using flow cytometry to measure the percentage of T cells present. c Dot plots representing CD3 + CD4 + cells. d Dot plots representing CD3 + CD8 + cells. f Quantification of percentage CD3 + CD4 + cells. g Quantification of CD3 + CD8 + cells. e Staining for CD8 in 8 dpi mouse brainstem tissue. Scale bar is 200 µm. Two-tailed Student’s t test was performed for statistical analysis (α = 0.05). ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001, ns, not significant. Source data are provided as a Source Data file.
