Fig. 1: The complete structure of the crenarchaeal RNA polymerase.
From: Structural basis of RNA polymerase inhibition by viral and host factors
a Cryo-EM map and cartoon representation of Sulfolobus acidocaldarius RNA polymerase. This composite map is illustrated as semi-trasparent surface and obtained by merging the cryo-EM map of apo-RNA polymerase with the stalk resulting from the multi-body refinement of the TFS4-bound RNA polymerase. Each subunit is highlighted according to the colour code shown on the left, the cryo-EM map is illustrated as semi-transparent surface. b Details of the apo-RNAP map showing the coordination system of the [3Fe-4S] cluster with sulphur atoms in yellow spheres and iron in dark red. Rpo3 residues are shown as sticks in red as in a, while the map is shown in mesh. c Cartoon representation of Rpo8 bound to Rpo1´ within the cryo-em map shown here in semi-transparency. The \({{{{\upbeta }}}}\)5–6 loop is highlighted in red, and the conserved GGLLM motif in orange. The corresponding sequence alignment is reported in Supplementary Fig. 4b.
