Fig. 1: Osteocalcin is induced in LRP1-depleted ECs.
From: Endothelium-specific depletion of LRP1 improves glucose homeostasis through inducing osteocalcin
a Heatmaps of mRNA-seq data demonstrate changes in gene expression profiles of liver ECs (MLivECs) and heart and lung ECs (MHLECs) isolated from LRP1 (eKO, LRP1f/f; Cdh5-CreER+/-) or their littermate control (WT, LRP1f/f; Cdh5-CreER-/-) mice. b A Venn diagram shows upregulated (UP) or downregulated (DOWN) gene numbers in LRP1-depleted MLivECs and MHLECs. c Expression changes of OCN and LRP1 were confirmed by real-time PCR. d Blood levels of Glu-, Gla- and total-OCN. e OCN1 and OCN2 mRNA levels in mouse lung ECs (MLECs) following transfection of LRP1 or control siRNAs. f OCN levels in conditioned media (CM) and whole-cell lysates (WCL) of osteoblasts (Ob) and different ECs. HUVEC, human umbilical vein endothelial cell. g OCN mRNA levels in marrow-flushed bone, osteoblasts (Ob) and ECs isolated from wildtype (WT) or LRP1 eKO mice. h OCN levels in CM of Obs and ECs isolated from WT or LRP1 eKO mice. n = 3 (c WT; c eKO, MHLEC), 4 (c eKO, MLivEC), 8 (d WT, Glu/Total-OCN), 7 (d Gla-OCN; eKO, Total-OCN), 6 (d eKO, Glu-OCN), 3 (e, g, h). NS, not significant. Data are presented as mean ± SEM. Analysis was two-way ANOVA followed by Fisher’s LSD multiple comparison test (c, g, h) or unpaired two-tailed Student’s t-test (d, e).
