Table 1 MIC values with acrB and oqxB complementation in E. coli BW25113.

From: Structure and function relationship of OqxB efflux pump from Klebsiella pneumoniae

Compounds

E. coli BW25113 (WT)

ΔacrAB

WT + Plasmid pTrc99a

WT + acrAB

WT + oqxAB

WT + oqxA + oqxB (R157A)

Linezolid#

400

12.5

400

200

>400

400

Novobiocin#

200

6.25

400

200

>400

200

Ciprofloxacin*

12.5

6.25

6.25

6.25

50

25

Levofloxacin*

25

6.25

12.5

12.5

200

25

Moxifloxacin*

25

12.5

25

25

>400

50

Rifampicin#

10

5

5

10

10

10

  1. #MIC values in µg/mL.
  2. *MIC values in ng/mL.
  3. E. coli BW25113 (WT: wildtype) or its ΔacrAB strain used for MIC.
  4. pTrc99a, +acrAB, +oqxAB, +oqxA + oqxB (R157A): complementation with plasmid carrying the respective genes.
  5. (Refer to Supplementary data for further details of the strains and constructs).
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