Fig. 5: Replication of SARS-CoV-2 in additional proposed host factors.

a Violin plot displaying the Log10 ratio between the viral titer at 48 h compared to 2 h post infection (p.i.) quantified by qPCR targeting the E gene in wildtype organoids and cells harboring a loss-of-function of a single host factor. Graphs display the ratio between viral titer at 48 h compared to 2 h post infection (p.i.). The dotted line indicates a fold change of 1. Dotted lines within the violins indicate the median and quartiles. N = 7 for WT, N = 3 for CEACAM1, DC-SIGN and MAVS, and N = 2 for the remaining genes. The WT data is redisplayed from Fig. 4a. b Model of host entry receptors and proteases involved in the entry of SARS-CoV-2. Essential and non-essential host factors based on the phenotypes in IO mutants are marked.