Fig. 6: Host protease dependency in the SARS-CoV-2 variant of concern B.1.1.7 and other coronaviruses.

a Violin plot displaying the Log10 ratio between the viral titer at 48 h compared to 2 h post infection (p.i.) quantified by qPCR targeting the N gene (SARS-CoV) or upE region (MERS-CoV) to measure viral replication in WT and TMPRSS2 KO organoids. DPP4 and ACE2 data include replication curves in Fig. 3C and S4A. The dotted line indicates a fold change of 1. Dotted lines within the violins indicate the median and quartiles. For MERS-CoV: N = 5 for WT, N = 2 for DPP4, N = 7 for TMPRSS2, N = 4 for ACE2, N = 3 for CTSL. For SARS-CoV: N = 5 for WT, N = 6 for ACE2, N = 7 for TMPRSS2, N = 4 for ACE2, N = 3 for CTSL. Exact p values can be found in Supplementary dataset 5. b Violin plot displaying the Log10 ratio between the viral titer at 48 h compared to 2 h post infection (p.i.) quantified by qPCR targeting the E gene to measure viral replication of the SARS-CoV-2 B.1.1.7 virus in intestinal organoid cells harboring a loss-of-function mutation in the TMPRSS2 and CTSL proteases, or ACE2. The dotted line indicates a fold change of 1. Dotted lines within the violins indicate the median and quartiles. N = 4 for ACE2 and N = 3 for the remaining genes. Statistics were performed by one-way ANOVA followed by a multiple-comparison test (Original FDR method of Benjamini and Hochberg; Q = 0.05) on log10 transformed values in a and b if N was ≥3. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. Exact p values can be found in Supplementary dataset 5.