Fig. 2: Cardiac RT persistently increases adult murine ventricular conduction.
a Representative ECGs from control (top) and IR mice (bottom) highlighting PR and QRS intervals. b, c Effect of radiation on PR (P = 0.39) and QRS (**P = 0.00043) intervals in control (black) versus IR (red) mice (n = 6 biologically independent animals per condition). d Representative ventricular activation maps from control and IR mice. Scale bars = 3 mm. e Effect of RT on ventricular CV (n = 6 biologically independent animals per condition; ***P = 0.00005; **P = 0.0034). f, g APD80 (P = 0.39) and ERP (P = 0.78) in control versus IR mice (n = 6 biologically independent animals per condition). h, i Western blots of NaV1.5 (*P = 0.022) and Cx43 (*P = 0.025) in control and IR ventricles (n = 3 biologically independent samples per condition). j Immunostaining of control (top) and IR (bottom) myocardium for NaV1.5 and k co-stained for Cx43 (red) and N-Cadherin (green). Scale bars = 70 μm. Experiment was replicated three times in biologically independent specimens and produced similar results. l, m Western blots of NaV1.5 (**P = 0.0034) and Cx43 (*P = 0.027) after 42 weeks post-IR (n = 3 biologically independent samples per condition). n Effect of RT on QRS intervals in control versus IR mice after 42 weeks (***P = 0.00027; C, n = 9; IR, n = 6). o Left ventricular CVs in control (n = 9 biologically independent animals) versus IR (n = 6 biologically independent animals) mice at 42 weeks post-IR (***P = 0.00042; **P = 0.0092). All P values determined by two-way unpaired t test. Bar graphs are represented as mean ± SD. Source data are provided as a Source data file.
