Fig. 5: SED1 tracks changes in osmolarity of a wide set of organisms. | Nature Communications

Fig. 5: SED1 tracks changes in osmolarity of a wide set of organisms.

From: Intrinsically disordered protein biosensor tracks the physical-chemical effects of osmotic stress on cells

Fig. 5

a Normalized FRET ratio (DxAm/DxDm) of live SED1-expressing Escherichia coli cells treated with different concentrations of NaCl. n = 3 independent experiments. One-way ANOVA. *p < 0.05, **p < 0.01, ***p < 0.001. Boxes represent 25th–75th percentile (line at median) with whiskers at 1.5*IQR. b Normalized FRET ratio (DxAm/DxDm) time course of Nicotiana benthamiana leaf discs transiently expressing SED1, treated with either water, 0.5 M NaCl, or 1 M sorbitol. n = 7–11 leaf discs. Mean ± SEM. One-way ANOVA. c Ratiometric image of live SED1-expressing U-2 OS cells at 300 mOsm (isosmotic) or 600 mOsm (hyperosmotic) treated with sorbitol. Scale bar = 50 μm. Calibration bar represents the normalized FRET ratio (DxAm/DxDm). d Normalized FRET ratio of SED1-expressing U-2 OS cells exposed to different osmotic treatments with sorbitol. n = 5 cells, 5 regions of interest per cell. One-way ANOVA. *p < 0.05, **p < 0.01, ***p < 0.001. Boxes represent 25th–75th percentile (line at median) with whiskers at 1.5*IQR. e Normalized FRET ratio of SED1-expressing U-2 OS cells exposed to different osmotic treatments with NaCl. n = 5 cells, 5 regions of interest per cell. One-way ANOVA. *p < 0.05, **p < 0.01, ***p < 0.001. Boxes represent 25th–75th percentile (line at median) with whiskers at 1.5*IQR. Source data are provided as a Source Data file.

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