Table 1 Kinetic and dissociation constants of LRRC8A-LRR domain-sybody interactions obtained by SPR.

From: Allosteric modulation of LRRC8 channels by targeting their cytoplasmic domains

  

KD (nM)

kon (mol−1 s−1)

koff (s−1)

Sb1

1

24

1.25E+06

0.030

 

2

22

1.66E+06

0.037

 

ave.

23

1.5E+06

0.034

Sb2

1

35

8.28E+04

0.003

 

2

60

4.99E+04

0.003

 

ave.

48

6.6E+04

0.003

Sb3

1

800

1.30E+05

0.104

 

2

913

1.23E+05

0.113

 

ave.

857

1.3E+05

0.109

Sb4

1

27

ND

ND

 

2

17

1.72E+06

0.064

 

ave.

22

-

-

Sb5

1

175

1.14E+06

0.199

 

2

170

1.43E+06

0.242

 

ave.

173

1.3E+06

0.221

  1. Data were fitted to a single binding site model. Table displays results of two independent biological replicates. Their average is shown in bold (ave.).
  2. The bold values display the average of the two biological replicates.
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