Fig. 1: Strategy, structures, and fluorescence properties of the PSMA-activatable probes.
From: A prostate-specific membrane antigen activated molecular rotor for real-time fluorescence imaging
a Schematic of probe binding and activation. b Structures, absorbance, and fluorescence spectra of PSMA activated fluorescent probes: Glu-490, ODAP-490, and ODAP-436. RFU relative fluorescence units. c Changes in fluorescence intensity of probes in solutions with increasing glycerol concentrations. Probe concentration was 0.1 mM and data were normalized to PBS control. Data are presented as mean values ± s.d. (n = 3 biologically independent experiments). d Inhibition of PSMA enzymatic activity using the radioenzymatic assay. Data are presented as mean values ± s.d. (n = 2 biologically independent experiments). e Saturation binding of rhPSMA/probe complexes. Data are presented as mean values ± s.d. (n = 3 biologically independent experiments). f Fluorescence intensity of the rhPSMA/probe complex in response to concentration changes. Data are presented as mean values ± s.d. (n = 3 biologically independent experiments). g Time frame for rhPSMA/probe complex formation. Data are presented as mean values ± s.d. (n = 3 biologically independent experiments). F.I. fluorescence intensity. Source data are provided in Source Data file.
