Fig. 6: Genital tract immunopathology is dependent on the P2X7 signaling pathway.

a ATP release from chlamydial infected BM12.4 cells. ATP released from infected cells was significantly increased throughout the course of infection (n = 9 per group). b ATP release from chlamydial infected BMDN. ATP released from CtD infected BMDN was significantly increased compared to CtD CT135⁻ infected BMDN cells at 2- and 4-h pi. c Secretion of IL-1β in BMDM derived from C57BL/6, NLRP3^−/−, Caspase1^−/−, and P2X7R^−/− mice, BMDM was primed with TNF-α (100 pg/ml) for 6 h and then treated with the supernatants from CtD, CtD CT135⁻, and CtD CT135⁻:: bla CT135 infected BMDN. Anhydrotetracycline hydrochloride (aTC) (50 ng/ml) was added to BMDN prior to and post-infection for the induction of CT135 (n = 3 per group). b, c Two independent experiments were performed. a–c, one-tailed student’s t test). d Infection of CtD infected C57BL/6 and P2X7R^−/− mice (n = 10 mice per group). e Infection kinetics of C57BL/6 and TNFα p55^−/−p75^−/−mice (n = 10 mice per group). f Quantification of gross uterine horn pathology at day 56 pi (n = 10 mice per group). g Flow cytometry of total CD11b⁺ F4/80⁺ macrophages in the whole GT of CtD infected mice at day 56 pi (n = 9 mice per group). h Flow cytometry of total CD11b⁺ F4/80⁺ Ly6C⁺ TNF-α⁺ macrophages in the whole GT of CtD infected mice (n = 10 mice per group). i Flow cytometry CD11b⁺ F4/80⁺ Ly6C⁺ TNF-α⁺ macrophages in panel (h). (d–h Two-tailed Mann–Whitney test). a–h Data are shown as the mean ± SD. a Data are pooled from three independent experiments. d–h Data are pooled from two independent experiments.