Fig. 1: N-terminomic analysis of SARS-CoV-2 infection of A549-Ace2 and Vero E6 cells. | Nature Communications

Fig. 1: N-terminomic analysis of SARS-CoV-2 infection of A549-Ace2 and Vero E6 cells.

From: Characterising proteolysis during SARS-CoV-2 infection identifies viral cleavage sites and cellular targets with therapeutic potential

Fig. 1: N-terminomic analysis of SARS-CoV-2 infection of A549-Ace2 and Vero E6 cells.

a Experimental design. b Viral RNA levels were determined by qRT-PCR (n = 3 biological replicates). c Protein levels were determined based on the TMTpro fractional intensity of the total protein intensity for the unenriched proteomic samples (n = 3 biological replicates). d Infectious virus production (PFU, n = 3 biological replicates). Error bars for b–d represent standard deviation. e A549-Ace2 and f Vero E6 neo-N-terminomic analysis reveals significant increases in peptides corresponding to viral and cellular neo-N-termini, where neo-N-termini must begin from amino acid 2 or later. P values were obtained by two-tailed unpaired t-test, correction for multiple-hypothesis testing to obtain Q-values was performed as described Storey (2002)70. bRP basic reverse phase fractionation, PFU plaque-forming units. The vertical and horizontal lines correspond to fold change (0.5) cut-off and Q-value (0.05) cut-offs, respectively.

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