Fig. 1: Hecw is preferentially expressed in fly gonads and central nervous system.
a Schematic representation of human HECW1 and Drosophila CG42797 proteins. Domains shown are: C2 (Ca2+ dependent lipid binding) domain in yellow, WW (substrate interacting) domains in green, and HECT (catalytic) domain in orange. The fly ortholog shows no C2 domain and presents an extended intrinsically disordered region (IDR), identified by IUPred (https://iupred2a.elte.hu/). Percentage of identity is reported below the domains. b Immunoblot (IB) analysis of the indicated Drosophila tissues performed with the anti-Hecw antibody. Ponceau shows equal loading. (n = 3). c IB of fly heads at 1, 30 and 60 days with the indicated antibodies. (n = 3). d Immunofluorescence (IF) analysis of ovarioles of 3-day-old flies performed with the indicated antibodies. Left panels: germarium and previtellogenic stages 1–7. Right panels: stage 10 egg chamber. Hecw colocalising with Orb (oocyte marker) at the posterior margin of the fly oocyte is indicated by white arrows and highlighted in the magnifications. Scale bar: 40 µm. (n = 3). e IF analysis of adult brains of 1-day-(upper panels) or 30-day-old flies (bottom panels) with the indicated antibodies. Neurons are marked with the anti-elav antibody and non-neuronal cells are circled in white. Scale bar: 5 µm. (n = 3).
