Fig. 4: Comparison of the ability of SQUIRREL and HAWKMAN to detect structural artefacts when faced with intensity differences in the input and reference images, on experimental high density data from the Localisation Microscopy Challenge.
From: Sub-diffraction error mapping for localisation microscopy images
a A pseudo widefield image produced by summing image frames. b SE and ME reconstructions. Blue arrows indicate areas of obvious sharpening compared to the widefield, the yellow arrow denotes an area of high intensity. c Error maps produce by SQUIRREL analysis show the largest errors (yellow) are dominated by the high intensity region in (a). The relative error is used instead of the confidence score to produce a HAWKMAN style confidence map (d) from the SQUIRREL data. When compared to the actual HAWKMAN result (e), it shows SQUIRREL principally detects the intensity differences and does not recognise the superior structural details (blue arrows) of the ME reconstruction. Scale bar 1 µm.
