Fig. 5: HAWKMAN can assess reconstructions from a variety of different structures.

Here, clathrin (a–d), tubulin (e–h) and the mitochondria epitope TOMM20 (i–l) have been imaged at medium-to-high density and evaluated with HAWKMAN. The test images were reconstructed using SE ThunderSTORM (a, e, i), and analysed with a reference image similarly reconstructed from the HAWK preprocessed dataset (b, f, j), and analysed using the sharpening map (c, g, k) and the confidence map (d, h, l). Data were displayed at a length scale of 32 nm (c, g) and 24 nm (k) for the sharpening maps, and at 96 nm (g, h) and 120 nm (l) for the confidence maps. The sharpening map threshold coefficients were 0.9 (c) and 0.7 (g, k). The confidence maps (d, h, l) highlight the substantial degrees of sharpening, particularly the TOMM20 reconstructions (i–l). Colouring as in Fig. 1, scale bars 2 μm.