Fig. 5: Comparison of biophysical and biological properties of TtgR variants.
From: Epistasis shapes the fitness landscape of an allosteric specificity switch
Ligand affinity (light bar) and EC50 sensitivity (dark bar) for resveratrol (a) and naringenin (b) are shown for TtgR variants 0000, 1000, 0100, 1100, and 1111. Ligand affinity was estimated by isothermal calorimetry and EC50 sensitivity from fitting dose–response curves to the Hill equation. EC50 values and error are calculated based on fitting to triplicate dose–response curves. ITC values and error are generated from a one-site binding model; error bars denote the error of the fit (see methods). The values shown are likely to underestimate the actual affinity and sensitivity values.
