Fig. 5: RPA overexpression rescues genomic instability in Discn KD mESCs. | Nature Communications

Fig. 5: RPA overexpression rescues genomic instability in Discn KD mESCs.

From: A novel lncRNA Discn fine-tunes replication protein A (RPA) availability to promote genomic stability

Fig. 5

RPA overexpression in Discn KD mESCs restored the ATR-CHK1 signaling (a), the stalled fork stability (b), and the fork restart ability (c). It also rescued the defects in dormant origin firing (d), ssDNA generation (e), and DSB formations (f) under HU treatment. In addition, RPA overexpression in Discn KD mESCs recovered the HR repair competence (g). Working model of Discn in safeguarding free RPA pool and genomic stability (h). Scale bar, 10 μm in (e) and 5 μm in (g). Three independent experiments were repeated with similar results in (a). At least 200 fibers from three independent replications were analyzed in (b, c). At least 50 continuous fibers from three independent replications were analyzed in (d). At least 19 images were analyzed in each condition in (e). Data were from three replications in (f, g). Data were representative of individual values with box and whiskers plots showing the median, upper and lower quartiles, and minimum and maximum in (d, e). Data were presented as mean ± SEM in (c, f and g). Statistical differences were determined using two-tailed Student’s t-test.

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