Fig. 3: Expression levels and kinetic activity of selected ATRs.
From: Machine learning-guided acyl-ACP reductase engineering for improved in vivo fatty alcohol production
a We measured the expression levels of the ATR-83 chimera and the three parental enzymes. These four enzymes displayed no significant differences in expression despite the large differences in their alcohol titers. The error bars represent one standard deviation centered at the mean (n = 3, 4, 3, and 2 for ATR-83, parent B, parent A and parent T, respectively, where n is the number of cultures analyzed, each from individual colonies). b We characterized the kinetics of selected enzymes on palmitoyl-ACP. The error bars represent one standard deviation centered at the mean (n = 4 technical replicates). ATR-83 displayed a higher turnover number (kcat) relative to parent B, and higher activity overall compared to the other parents. The kinetic parameters for parents A and T could not be precisely determined due to their low overall activity and the resulting poor fit to the Michaelis–Menten model. Source data are provided as a Source Data file.
