Fig. 7: SON depletion leads to suppression of proliferation, sphere formation, and clonogenic ability of GSCs, and these effects are facilitated by hnRNP A2B1 knockdown but abrogated by RBFOX2 knockdown. | Nature Communications

Fig. 7: SON depletion leads to suppression of proliferation, sphere formation, and clonogenic ability of GSCs, and these effects are facilitated by hnRNP A2B1 knockdown but abrogated by RBFOX2 knockdown.

From: SON drives oncogenic RNA splicing in glioblastoma by regulating PTBP1/PTBP2 switching and RBFOX2 activity

Fig. 7: SON depletion leads to suppression of proliferation, sphere formation, and clonogenic ability of GSCs, and these effects are facilitated by hnRNP A2B1 knockdown but abrogated by RBFOX2 knockdown.

a Cell growth rate was determined by MTT assay of GSC #83 expressing indicated shRNAs (n = 5). b, c Representative phase-contrast images of the GSC spheres (b) and a graph indicating sphere numbers (c, n = 3) formed by the GSCs with the knockdown of the indicated genes. Images are representative of n = 3 independent experiments. Yellow bars, 50 µm. d Alkaline phosphatase assays were conducted by nitro blue tetrazolium chloride (NBT) staining with GSCs to assess the effect of knockdown of the indicated genes in GSC sphere formation. Representative images of NBT-stained spheres (n = 3) viewed as a whole plate (left) and the magnified images (right). Yellow bars, 50 µm. e The histogram indicates the number of NBT-positive spheres (n = 3). Error bars in all graphs represent the standard deviation (SD) of tests. NS not significant, *p < 0.05, **p < 0.01. Statistical significance was determined by an unpaired two-tailed t-test. Source data are provided in the Source data file.

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